Device

Part:BBa_K1329001:Design

Designed by: Daniela Geist   Group: iGEM14_Marburg   (2014-10-01)


Hag-KpnI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 588


Design Notes

The position of the KpnI site had to be well placed in order to provide misfolding and structural problems of the flagellin which could influence the assembly of the filament.


Source

The sequence for this part comes from the Bacillus subtilis genome.

References